Transition Elements And Coordination Compounds Biology Essay

Transition Elements And Coordination Compounds Biology EssayAs we know, Manganese is set in the prime(prenominal) row of transition metal with the electron abidance Ar 3d5 4s2. Besides that, Manganese has different type of oxidation states when it appears as a fuse and the oxidation state is from Mn(-III) until Mn(VII). So, we know that the compounds of manganese range in the oxidation twist acquit a different of 10 electrons. In the experiment 1, we prep be tris(acetylacetonato)manganese(III), Mn(acac)3 by utilise manganese(II) chloride tetrahydrate and potassium permanganate act as oxidation agent to oxidise manganese(II) chloride to acetyl propanonemanganese(III).Manganese(III) acetylacetonate is an one- electron oxidant. Manganese(III) acetylacetonateis high spin. It has besides a distorted octahedral composite plant body part. This distortion is due to the Jahn-Teller effect. (Absolute Astronomy, 2009). The structure of Manganese(III) acetylacetonate is shown as below- (Source Tcieurope.com)The comparison is as get hitched with-MnCl2 + 4 water Mn( urine)4Cl2Mn(H2O)4 Cl + 2HC5H7O2 + NaC2H3O2 Mn(C5H7O2)2 + NaCl + HC2H2O24Mn(C5H7O2)2 + KMnO4 + 7HC5H7O2 + HC2H3O2 5Mn(C5H7O2)3 + KC2H3O2 + 4H2OFurther more(prenominal), bis(acetylacetonato)oxovanadium(IV) is also know as Vanadyl acetylacetonate, VO(acac)2. As we know, it is a fat green Gordian. bis(acetylacetonato)oxovanadium(IV) has a vanadyl group, VO2+. The vanadyl group is beated to 2 acetylacetonate anions and the structure of the compound is as celebrate-This complex ignore be make from vanadium(IV) or vanadium(V). In our experiment, bis(acetylacetonato)oxovanadium(IV) was prep atomic number 18d from vanadium(V) oxide and the par is as review-V2O5 + 2H2SO4 + EtOH 2VOSO4 + 3H2O + CH3CHOVOSO4 + 2HC5H7O2 + Na2CO3 VO(C5H7O2)2 + Na2So4 + H2O + CO2(Absolute Astronomy, 2009)Besides that, both of the acetylacetonato (acac) groups of bis(acetylacetonato)oxovanadium(IV) ar able to be exchange with organic ligands having coordinating atoms of different potentialities. (Maurya, 2003)Both Manganese(III) acetylacetonate and bis(acetylacetonato)oxovanadium(IV) be link with acetylacetonate which known as ligand. The precursor for acetylacetonate is acetylacetone with framingula C5H8O2. However, acetylacetonate is an anion. It fag bind to equivalent cation but it very hard to exist as a bountiful ion in solution.In addition, Cobalt is a hard, gray metal. It has a proton number 27. Besided that, there ar two types of cobalt ions namely Co2+ and Co3+. First, Co3+ ion is more shorter than the Co2+ ion. However, the complex ion formed with higher oxidation state is more stable. ( zmir get of Technology, n.d.). So that, Cobalt(III) complexes are kinetically inert.Co3+ can undergo a offset known as ligand exchange reactions slowly which compared to Co2+ complexes. The cobalt(III) complexes are usually in octahedral shape. In the experiment, chloropentaamminecobalt(III) chlor ide is being synthesized. The structure is as follow-(Source Chemicalbook.com)The complex is prepared by the oxidation of ammoniacal solution of cobalt(II) salts by using hydrogen peroxide. The formula is as follow-Co2+ + NH4+ + 1/2H202 Co(NH3)5H203+Co(NH3)5H203+ + 3Cl- Co(NH3)5ClCl2 + H20( zmir Institute of Technology, n.d.)Materials and Methods Experiment one5g of MnCl2.4H201.3g of NaC2H3O2.3H2O NaC2H3O2.3H2O change state in 200cm3 of distilled water.21cm3 of 2HC5H7O2 slowly added1g of KMnO4Present of two-phase social class upshot A added in with stirringDissolved in 50cm3 of distilled water.13g of NaC2H3O2.3H2OSolution B added inSolution ASolution BDissolved in 50cm3 of distilled water.Heated with 60oC for 30 minutesComplex washed with acetoneSolid complex filtered by suctionResultant solution was cooled with ice-cold waterExperiment 2Experiment 3RecrystalliseResults For experiment 1,from the equation below, I can get the theoretical potful of the Mn(acac)3 substantial comp lex by Mn(H2O)4 Cl + 2HC5H7O2 + NaC2H3O2 Mn(C5H7O2)2 + NaCl + HC2H2O24Mn(C5H7O2)2 + KMnO4 + 7HC5H7O2 + HC2H3O2 5Mn(C5H7O2)3 + KC2H3O2 + 4H2OFrom the equation, we know that 1 breakwater of Mn(H2O)4 Cl = 1 groyne of Mn(C5H7O2)2.So, 5 g of Mn(H2O)4 Cl = 0.0308 mol is also = 0.0308 mol of Mn(C5H7O2)2.From the second equation, 4 mol of Mn(C5H7O2)2 = 5 mol of Mn(acac)30.0308 mol of Mn(C5H7O2)2 = 0.0385 mol. Of Mn(acac)3So, theoretical free metric weight unit of Mn(acac)3 = 0.0385 mol X 252.938 g/moltheoretical weight of Mn(acac)3 = 9.7381 gThe following shows the manner to get our data-based weight slant of hear tube14. 8180 gWeight of Sample tube + steady complex , Mn(acac)318.7785 gSo, the experimental weight of Mn(acac)3complexes were 3.9605 gPercentage kick in of Mn(acac)3complexes we get was = 3.9605 g / 9.7381 g X coulomb %= 40.67 %Next, magnetized arcminute of Mn(acac)3complexes were calculated as follow m = 0.9278g- 0.8193g= 0.1085gRo= -33L = 2.4cmR= 1165 (paracharisma tic)CBal = 1X(g) = CBal X L X (R-Ro) / 109 X mX(g) of Mn(acac)3complexes = 2.65 X 10-5So, Mn(acac)3complexes are paramagneticFTIRInterpretation of IR spectrum for complexes allow be written in discussion.For experiment 2,from the equation below, I can get the theoretical mass of the Co(NH3)5ClCl2 square complex by Co2+ + NH4+ + 1/2H202 Co(NH3)5H203+Co(NH3)5H203+ + 3Cl- Co(NH3)5ClCl2 + H20From the above equation, 1 mol of Co2+ = 1 mol of Co(NH3)5H2O3+12g of Co2+ = 0.0504 molSo 0.0504 mol of Co(NH3)5H203+= 0.0504 mol of Co(NH3)5ClCl2Theoretical weight of Co(NH3)5ClCl2 solid complexes = 0.0504 mol X 250.433 g/mol= 12.6218 gThe following shows the method to get our experimental weightWeight of Sample tube14. 9285 gWeight of Sample tube + solid complex ,22.3723 gSo, the experimental weight of Co(NH3)5ClCl2 solid complexes = 7.4438 gPercentage yield of Co(NH3)5ClCl2complexes = 7.4438 g / 12.6218 g X 100 %= 58.98 %Next, magnetic moment of Co(NH3)5ClCl2complexes were calculated as fol low m = 0.9264g- 0.8207g= 0.1057gRo= -36L = 2.1cmR= -41 (dimagnetic)CBal = 1X(g) = CBal X L X (R-Ro) / 109 X mX(g) of Co(NH3)5ClCl2complexes = -9.9338 X 10-8So, Co(NH3)5ClCl2complexes are diamagneticFTIRFor experiment 3,from the equation below, I can get the theoretical mass of the Vo(acac)2(H2O) solid complex by V2O5 + 2H2SO4 + EtOH 2VOSO4 + 3H2O + CH3CHOVOSO4 + 2HC5H7O2 + Na2CO3 VO(C5H7O2)2 + Na2So4 + H2O + CO2From the above equation, 1 mol of V2O5 = 2 mol of VOSO42g 0f V2O5 = 0.011 mol = 0.022 mol of VOSO42 mol of VOSO4 = 2 mol of VO(C5H7O2)2Theoretical weight of VO(C5H7O2)2= 0.022 mol X 264.94 g/mol= 5.8287 gThe following shows the method to get our experimental weightWeight of Sample tube14.8445 gWeight of Sample tube + solid complex ,18.5818 gSo, the experimental weight of VO(C5H7O2)2= 3.7373 gPercentage yield of VO(C5H7O2)2= 3.7373 g / 5.8287 g X 100%= 64.12%Next, Magnetic moment of bastardised VO(C5H7O2)2 complexes were calculated as follow m = 0.8880g 0.8244 g= 0.0636gRo = -34L = 2.5cmR= 72 (paramagnetic)CBal = 1X(g) = CBal X L X (R-Ro) / 109 X mX(g) of impure VO(C5H7O2)2complexes = 4.17 X 10-6So, VO(C5H7O2)2complexes are paramagneticMagnetic moment of pure VO(C5H7O2)2 complexes were calculated as follow m = 0.8947g -0.8211= 0.0736gRo= -33L = 2.3cmR= 144 (paramagnetic)CBal = 1X(g) of pure VO(C5H7O2)2complexes = 5.53 X 10-6FTIRInterpretation of IR spectrum for complexes will be written in discussion. dirty VO(C5H7O2)2Pure VO(C5H7O2)2Discussion Interpretation of IR spectrum for tris(acetylacetonato)manganese(III)Wavenumber (cm-1)Description of bands2921.1 2959.7-relative passion vagueCH stretchability of CH31593.91508.0-relative fanaticism toil rough-(C=C) stretchiness-(C=CH) distorted shape1387.2-relative mass salutary-(CH3)- symmetric C-H deformation1253.5-relative inspiration strong-(C=C) reaching-(C-CH3) stretching1016.8-relative intensity strong-(CH3) out-of plane bending923.2-relative intensity strong-(C-CH3) stretching777.0-r elative intensity strong-(C-H)deformation678.1-relative intensity f oxygenize/ strong-(C-CH3)stretching,(O=C-CH3) deformation-(Mn-O) stretching indicates metal-ligand bewilder460.1 relative intensity weak (C=C) stretching,(C-CH3) stretching-(Mn-O) stretching that also indicatesmetal-ligand shackleInterpretation of IR spectrum for chloropentaamminecobalt(III) chlorideWavenumber (cm-1)Description of bands3258.0-relative intensity strongNH3stretch1576.2-relative intensity fair-degenerate asymmetric NH3stretching1307.8-relative intensity strong-symmetric NH3angle deformation844.9-relative intensity strong-NH3rocking487.6-(Co-Cl) stretching indicates metal-ligand trammel netInterpretation of IR spectrum for impure bis(acetylacetonato)oxovanadium(IV)Wavenumber (cm-1)Description of bands1556.81521.0-relative intensity medium (C=O) stretching-( C=C),(C=CH) stretching1418.7-relative intensity medium-(CH3) deformation1374.01357.6-relative intensity strong-(C=O) stretching-(CH3) deformation mode1286.6-relative intensity strong-(C=C=C) stretching998.2-relative intensity strong and sharp-stretching of V=O bond-it also indicates the metal-ligand bond.1018.6-relative intensity strong-(CH3) rocking936.1-relative intensity strong-(C-CH3) stretching-(C=O) stretching798.6-relative intensity medium-(C-H) out-of-plane bending685.9657.2-relative intensity medium/ weak-(ring) deformation out-of-plane bending for609.2-(ring) deformationInterpretation of IR spectrum for pure bis(acetylacetonato)oxovanadium(IV)Wavenumber (cm-1)Description of bands1563.01520.0-relative intensity medium (C=O) stretching-( C=C),(C=CH) stretching1499.6-relative intensity medium-(CH3) deformation1380.01349.0-relative intensity strong-(C=O) stretching-(CH3) deformation mode1288.3-relative intensity strong-(C=C=C) stretching995.0-relative intensity strong and sharp-stretching of V=O bond-it also indicates the metal-ligand bond.1018.2-relative intensity strong-(CH3) rocking935.7-rela tive intensity strong-(C-CH3) stretching-(C=O) stretching798.8798.0-relative intensity medium-(C-H) out-of-plane bending686.0-relative intensity medium/ weak-(ring) deformation out-of-plane bending for609.7-(ring) deformationFTIR is known as Fourier Transform invisible Spectroscopy. This FTIR can be use to identify different types of chemical bond which is either organic compound or inorganic compound. So no two different compounds will have same spectrum. However, FTIR may earn destructive to our sample compare to magnetic susceptibility which is non-destructive. Besides that, magnetic susceptibility can be group by paramagnetic, diamagnetic and ferromagnetic. paramagnetic substance is those attracted by strong magnetic palm but those repelled by magnetic field are diamagnetic substances.Besides that, we can also confirm the shape by using magnetic susceptibility. The Cobalt ion to form Chloropentaamminecobalt(III) chloride has 6 electron in d orbital. According to crystal f ield theory, if the complex is in octahedral shape, the electron can be arranged worry below-Low-spin high-spineg egt2g t2gIf the complex is in tetrahedral shape, the electron are arranged as below-t2gegMagnetic Susceptibility for chloropentaamminecobalt(III) chloride is -9.9338 X 10-8 and it is diamagnetic. Only the low-spin octahedral shape shows diamagnetic properties, so the shape of chloropentaamminecobalt(III) chloride is octahedral.Furthermore, for tris(acetylacetonato)manganese(III), the Mn3+ ion has 4 electron in d orbital, and the correspondence in octahedral shape will as follows-Low-spin High-spineg egt2g t2gFor the arrangement in tetrahedral shape, the electrons are arranged as below-t2gegMagnetic Susceptibility for tris(acetylacetonato)manganese(III) is 2.65 X 10-5 and it is paramagnetic. So the shape of tris(acetylacetonato)manganese(III) is octahedral. However we cannot determine whether is low-spin or high-spin in this situation because we are not calculating the pairing energy for the complex.In addition, there is solo one electron in d orbital for vanadium ion of bis(acetylacetonato)oxovanadium(IV). Magnetic susceptibility for it is 4.17 X 10-6 and it is paramagnetic. The shape is octahedral and the arrangement of electron is as follow-egt2gAcetylacetonate is delocalized and formed resonance structure as follow The structure of Co(NH3)5ClCl2 is as follow The structure of VO(ACAC)2 is as follow Moreover, oxovanadium complexes act as insulin mimetics, nucleolytic and antineoplastic.To separate insulin- mimetic complexes, a simple and fast in-vitro assay is developed. Besides that, an holy assessment of the cells taken up of glucose, in-vitro assay with Ehrlich can be used. The oxovanadium complexes can cleave DNA without the present of hydrogen peroxide. Then, its nucleolytic efficiency is also great but it is affected by the choice of buffer and pH. The oxovanadium complexes is also an anticancer agent against human ovarian cancer.Actu ally, I have acquired a volume of new knowledge among these three experiments. First, experiment one and two are easier to carry out compared to experiment three. This is because experiment one and two multiform oxidation which Mn2+ was oxidized to Mn3+ Co2+ was oxidized to Co3+. Whereas experiment 3 knobbed reduction which reduced VO5+ to VO4+. At the beginning of each experiments, we heated up the sample in open air. It is easier to oxidize the compound than reduce the compound in the presence of oxygen. So, we need more time to heated the V2O5.During the first time of experiment three, I failed to get a blue tinge solid powder. This is because I heated the sample in the open air and I failed to control the heater. So, after the filtration process, I got a white solid powder. Therefore, I learnt from the mistake and during the second time I used reflux method. This time I can control the heating process well. The grain alcohol was not evaporated much. After filtration, I got sort of a lot of blue solid powder. So, I knew that, for reduction process, we cannot heat the sample too pronto and heat too long. If we heated too long, most of the product will evaporate.Next, from the FTIR spectrum, I can observe that the spectrum from the first and third experiment are quite similar. This is because, the peak from the spectrum are mostly from the acetylacetonato (acac). However, spectrum from experiment two is quite different from otherwise experiment because mostly the peaks come from Cl compared to other experiments.The percentage yield of the complexes from all the three experiment that I have calculate out was not so high. This is by chance due to the dry wash of the product with acetone, slightly of the product was dissolved and washed away. However, there are other factors which cause problem to our results. First, most of the heating plate we used cannot function well. I changed many heating plate during the experiment. Next, we have unbroken our half way done sample for more than 7 days. The results may not be so accurate anymore. Another factor maybe due to the volume and amount of the sample and other material we pecker are not accurate. Our product may contain some impurities.However, some of the precaution was taken during the experiment. In these experiments, some of the chemical was corrosive and hurtful such as concentrated HCl and H2SO4. So, we poured the chemical carefully in the smoking board. Next, before using any instrument, we read through the manual to transform the steps to function the magnetic susceptibility balance. We also taken knock off some important steps to function the FTIR spectrophotometer. I have asked some help from lab assistant on where to get the material for experiment. closing The percentage yield for tris(acetylacetonato)manganese(III) is 40.67%, chloropentaamminecobalt(III) chloride is 58.98% and (acetylacetonato)oxovanadium(IV) is 64.12%.For tris(acetylacetonato)manganese(III), the peak for two Mn-O bond are at the region approximately 678.1 cm-1 and 458.3 cm-1. For chloropentaamminecobalt(III) chloride, the peak for Co-N bond is at 669.2 cm-1 while Co-Cl bond is at 486.2 cm-1. For bis(acetylacetonato)oxovanadium(IV), the peak for V=O bond is at 997.4 cm-1 region.Magnetic Susceptibility for tris(acetylacetonato)manganese(III) is 2.65 x 10-5 and it is paramagnetic. Besides, Magnetic Susceptibility for chloropentaamminecobalt(III) chloride is -9.93x 10-8 and is diamagnetic. For bis(acetylacetonato)oxovanadium(IV), magnetic susceptibility for impure complex and pure complex are 4.17X 10-6 and 5.53X 10-6 respectively. They are paramagnetic.